Devendra K. Chauhan                            

Rice Biotechnology Lab
CSSRI, Karnal,                             
Haryana, India                                                                    
Email , chouhan1971@yahoo.co.in / chouhan@lycos.com 
+91 98964 52662  (Mobile)
                                               
Presently working as Research Associate under the project “Transgenics in Crops- (Functional genomics Component)” at Central Soil Salinity Research Institute (CSSRI), Karnal, India and involve in the following activities:
-  Polymorphism survey using SSR and SNPs markers
-  Phenotyping of RILs for salt tolerance under salt stress and normal conditions
-  Genotyping of RILs using SSR and SNPs markers
-  Fine mapping of QTL regions for salinity tolerance and identification of probable candidate genes
-  PCR amplification of candidate genes and RNA expression profiling of the genes in the segregating lines
-  Proteomics analysis of root and shoot extracts for extraction profiling of cultivar set and RILs
-  Map based cloning of major QTLs and its validation through transformation and association genetics.
 
Work at NRC for Soybean
I have completed molecular screening of salt tolerant and susceptible soybean genotypes using SSR markers, Molecular screening of Indian soybean varieties and some mutation lines through microsatellite markers (STMS) and worked on gene tagging and mapping for Yellow Mosaic Virus through Bulk Segregation Analysis (BSA). Characterization of wild relatives of soybean by microsatellite markers and molecular characterization of soybean germplasm collected from U.S.A., China, Japan, Taiwan and India (manuscript under preparation) in molecular biology laboratory at National Research Centre of Soybean, Indore, M. P. INDIA.
 
Work at NBPGR
Earlier I worked as Research Associate at National Bureau of Plant Genetic Resources New Delhi. I was involved in micro-propagation and In-vitro conservation of temperate (Apple, Peach, Plum), under utilized fruits (Aegel marmelos, Zizyphus mauritiana and Punica granetum) and tropical aroids (Alocasia, Collocasia and Xanthosoma sp.).
 
Techniques known
¨      Expertise in tissue culture techniques
¨      High frequency plantlet regeneration using different explants
¨      Somatic Embryogenesis
¨      Cryo-preservation
¨      Isolation, Purification and Quantification of total genomic DNA.
¨      Expertise in Molecular techniques (RFLP, RAPD and SSR)
¨      Estimation of different salt composition in leaf and roots tissue extracts
¨      Biochemical techniques like isozyme analysis
¨      Familiar with MS office, software related to analysis of molecular data e.g. NTSYS and MAPMAKER etc.
 
Field of Interest:
I have keen interest to work in field of Genetics and Plant Breeding through conventional breeding techniques. Equally interested in new tools of molecular plant breeding viz. tagging, mapping, Isolation, cloning and sequencing of genes for desirable traits and marker assisted breeding and genome analysis by using latest bioinformatic tools. I am also interested to work on vector construction and different modes of transformation of plant tissues by using Agro bacterium and biolistic methods.
 
Training attended:
1. Attended lecture classes on advanced training “Molecular approaches in crop    improvement” Conducted by International Center for Genetic Engineering and     Biotechnology (ICGEB) New Delhi from 11-22 Nov. 2002. Following topics were covered in detail under this training programme:
Ø  Mapping and tagging for gene of interest
Ø  Marker aided selection
Ø  Methods for plant transformation
Ø  Genetic engineering for biotic and abiotic stress
Ø  Improvement for nutritional quality
 
· Attended Two days training on Protection of plant varieties and farmers act in relation to soybean conducted by NRC for soybean Indore, MP, India, from Oct 5-6th 2005.
Conference attended:
v  Attended “IInd International Rice Congress” held at New Delhi-12, Oct 9-13, 2006.
v  Attended “XIIth National Seed Seminar” held at ANGRU campus Hyderabad, India Feb. 25-27, 2006.
v  Attended 88th session of “Indian Science Congress” held at New Delhi-12, Jan. 6-9, 2001.
v  Attended “International Conference on managing Natural Resources” Held at New Delhi during Oct. 14-18, 2000.

Employers’ Address:

Educational Qualification:

M.Sc. Thesis title: “Impact of time of planting on seed and plant characters in spring bread wheat ( Triticum aestivum}”.
Ph. D. Thesis title: “Identification of rice genotypes using morphological characters and biochemical markers (RAPD).”
 
Fellowships:
¨      ICAR merit scholarship during B. Sc. (Ag & AH.)
Professional Examination passed:
v  Qualified National Eligibility Test (NET, 1999) conducted by Agricultural Scientist Recruitment Board (ASRB) ICAR.
Personal Information
Fathers’ Name:                                    Sh. Iqubal Singh
Permanent Address:                            Village & P.O. Ramala
                                                      Distt. Baghpat, U.P., India
Date of Birth:                                      01-01- 1971
Phone Number:                                   098964 52662
Nationality:                                         Indian
 
Research Publication:
Ø   John Milton Lima, Abhishek Dass, S.C. Sahu, L. Behera and Devendra K.    Chauhan “A RAPD marker identified a susceptible specific locus for gall midge resistance gene in rice cultivar ARC5984” paper accepted in Crop Protection
 
Ø  C. Tara Satyavathi, Monika Sharma, Devendra K. Chauhan, Ch. Bhardwaj, G. S. Chauhan “Use of STMS Markers for varietal identification in soybean”, Communicated to Indian J. of Genetics and Plant Breeding
 
Ø  R.K. Singh, R.K. Raipuria1, V.S. Bhatia, Anita Rani, Pushpendra, S. M. Husain, Devendra K. Chauhan, G.S. Chauhan and T. Mohapatra “SSR Markers Associated with Seed longevity in Soybean” Accepted in Seed Science and Technology
 
 
Ø  R K Singh, Devendra K. Chauhan and N. Maharolia  “Genetic diversity among soybean genotypes of USA, China, Japan, Taiwan and India as revealed by SSR markers”. Accepted in  Plant Genetic Resources and Crop Evolution
 
Ø  R. K. Singh, Anita Rani, Devendra K Chauhan, K. V. Bhat, P. Dohare, V. Kumar and C. Tara Satywathi “Molecular genetic and physio - morphological evaluation of soybean cultivars differing in response to sodium chloride sensitivity”  Communicated to Euphytica
 
Ø  Chauhan D.K., A. Dass and S. K. Malik, High frequency direct plantlet regeneration using leaf explants in Garcinia indica: An important medicinal plant Communicted to Plant Science
 
Ø  Devendra K. Chauhan “Molecular characterization of Indian Soybean genotypes using STMS markers (Manuscript under preparation).
 
Ø  Chauhan D.K., S. S Malik and S. P. Singh, Genetic diversity studies and cluster analysis in rice. Communicated to Indian J. of Plant Genetic Resources
 
Ø  Chauhan D.K., S. S. Malik, S. P Singh, Identification of rice genotypes using RAPD markers. Communicated to Crop Research
 
Ø  Chauhan D.K., S. S. Malik and S. P. Singh “Heritability and correlation studies in           rice”. Communicated to Maharashtra J. of Agricultural Science
 
Oral Presentation:
v  Tara Satyavathi, Monika Sharma, Devendra K. Chauhan, Ch. Bhardwaj, R.K. Singh, S. M. Hussain, G. S. Chauhan (2006) “Use of STMS Markers for Varietal Identification” Poster to be presenting at “XIIth National Seed seminar” on Prosperity through seeds” held at ANGRAU Campus Hyderabad from Feb. 23-25, 2006.
 
Posters Presented:
R.K.Gautam, Shiv Ratan, A.K. Nayak, D.K. Sharma, A. Qadar, D. Chauhan and A. Dass (2007) Harnessing inter and intra-specific tolerance in medicinal plants to rehabilitate sodic soils International Conference on Sustainable Agriculture for Food, Bio-energy and Livelihood Security held at JNKVV Jabalpur, M.P. India on Feb.14-16,
 
Devendra K. Chauhan; Efficient and rapid micro propagation in Kokam (Garcinia indica) Poster presented at National Conference on “Advancing frontiers in biotechnology for sustainable Agriculture and Health” held at Bareilly College Bareilly, April 28-29, 2005
 
Devendra K. Chauhan, S.S.Malik, S.P.Singh, Genetic diversity studies in rice based on morphological and molecular markers. Poster presented at National Conference on “Advancing frontiers in biotechnology for sustainable Agriculture and Health” held at Bareilly College Bareilly, April 28-29, 2005
 
Sanjeev Saxena A.B. Chaudhary, Devendra K. Chauhan (2001). Management of germplasm collected under NATP project. Indian J. of Plant Genetic Resources Special issue.
Mandal B.B. and  Devendra K. Chauhan  (2000) In-vitro conservation of tropical            aroids. “International conference on managing Natural Resources for Sustainable Agriculture Production in 21st century” Held at New Delhi at Feb 14-18, 2000
Chauhan D.K., Sohan Pal, D.P. Saini and S. K. Malik (1997) Roll of seed technology      in sustainable yield potential of wheat through quality seed. “Proceedings of International group meeting on wheat needs beyond 2000AD”. International conference on sustainable yield potential in wheat 21st century held at DWR Karnal August 12-14, 1997.
               
References

Dr N.K. Singh
Principal Scientist (Biotechnology)
NRC on Plant Biotechnology
IARI Campus, Pusa, New Delhi-12
nksingh@nrcpb.com
 
Dr S. M. Husain
Head, Crop Improvement Section
National Research Center for Soybean
Khandwa Road Indore, M. P.India
E-mail: smhusain2@yahoo.com
Ph: {0} 0731-2362835, Ext-124
{R} 0731-2762898
 
Dr R. K. Singh
Senior Scientist, (Biotechnology)
Indian Institute of Sugarcane Research,
Lucknow, U. P.India
Ph. 094509 30028
Email: ikrps@yahoo.com

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