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R. RADHA KRISHNA
FLAT NO. 404, SRI SAI KRUPA RESIDENCY
TEMPLE ALWAL, SECUNDERABAD – 10,
ANDHRA PRADESH, INDIA
E mail.
krishnaradha_k@rediffmail.com;
raadhaakrishna@gmail.com
Phone: 91-040-27974789
Cell : 9441876976
OBJECTIVE: Work as Top/Middle management for seed
industry R & D wing in Biotechnology or Knowledge Out Sourcing
(KPO) Centre
WORK EXPERIENCE: 18 YEARS (Total Experience in Different
Positions)
STUDY DETAILS
Master of
Science M.Sc., (Applied Botany & Biotechnology)
University : Gauhati University, Gauhati, Assam State, India
Year of Pass : 1992 (1989-1991)
Thesis in M.Sc.. : Study of Sister Chromatid Exchanges in
Anesthesiologists (Medical Genetics/Biotechnology).
Bachelor of Science : Bachelor or Science B.Sc., (Botany,
Zoology & Chemistry)
University : Andhra University, (Hindu College,
Machilipatnam), AP, India.
Year of Pass : 1981 (1978-81)
SKILLS
Managerial Skills:
1. Take over all responsibility to establish good report
between management and technical staff for achieving targets
and dead lines. Responsibility to take any challenging jobs
to achieve with rational approach
2. Preparation and project and implementation and
coordination to complete the task
3. Staff selection, training and coordinating to put them in
correct stream for good outcome.
4. Involve in all related sectors as team member to plan and
monitor to finish the task in time.
5. Involve in lab instruments purchase and installation the
same, plan for maximum usage.
Other Skills:
Excellent understanding of or ability to attain and
maintain a working knowledge of Good Clinical Practices
Basic computer skills with Word, Excel and Power point
presentation
Understanding of medical terminology with basics\
Excellent oral and written communication skills with
ability to communicate with medical personnel
Proven flexibility and adaptability
Able to work in a team environment and independently
Cygnus Experience (work profile)
1. Preparation of industry insights and QPACs for Services
and manufacturing sectors as a team leader with more than 15
members
2. Coordination, supervision and give expert comments for
industry monitor modules like company scan, product scan,
stock scan, news and events in life sciences and
manufacturing industries
3. Training MBA students for summer program, industry
monitors preparation and their SIP
4. Planning and monitoring the progress of industry monitors
status for meet deadlines
5. Motivation to students and Cygnus staff to meet the
targets
Laboratory Techniques:
1. Recombinant DNA Technology for genomic DNA : RAPD, RFLP,
AFLP, DNA Finger Printing for ownership claim, Ligation,
Disgestion of DNA with different restricted enzymes,
Southern Blotting, Northern Blotting, Identification and
Isolation of Genomic Markers, and its Sequencing,
2. Protein Profile : Isolation of seed proteins for
establishment of protein profile for genetic studies,
estimation of genetic purity of seed lots.
3. Cotton Testing Laboratory: Cotton Fibre length
estimation, strength estimation and estimation of genning
percentage, cotton oil estimation etc.
4. Seed Testing Laboratory : Conducting Physical purity of
seeds, germination testing, moisture testing and seed
viability testing.
Field Techniques:
1. Field trials : Conducing Screening germplasm, Conducting
All India Coordinated Trials for various crops like (cotton,
soybean, sunflower, Rice, maize) etc.
2. Multi location trials : Conducing cotton, soybean,
sunflower, maize and coordinating with marketing team for
MLTs and MATs (Market acceptable tests)
3. Evaluation trials: Conducing Different crops including
vegetable crops for yield and phenotypic characters
identification.
4. Intercrop Cultivation: Conducting evaluation trials for
interecrop Cotton with soybean for identification of higher
yield varieties in soybean.
Administration:
1. Correspondence, over all incharge responsibility for
Biotechnology lab administration
2. Addressing Day to day problems
3. Planning and Fixing delivery targets
4. Motivation of employees for optimum results
5. Technical Advice/guidance to colleagues and management in
Research activities
Techniques standardized in the Laboratory
Extraction of Genomic DNA from Bacteria, Plant material
and human blood, purification and quantification
Extraction of mRNA for cDNA preparation and analysis.
PCR Amplification of Genomic DNA for RAPD and AFLP
Southern Blotting and Hybridization with Probe for RFLP
Ligation of DNA with Plasmid
Preparation of Competent Cells
Micro Plating and cell culture
SDS PAGE Analysis for Proteins Banding pattern.
Major Imported Instruments Handled So Far:
PCR Machine
Electrophoresis Units – Vertical and Horizontal
Transilluminator and Gel Documentation Unit
Spectrophotometer – Perkin Elmer – (Bio-Lambda) for DNA
quantification analysis.
Ultra centrifuges,
All miscellaneous instruments related to Biotechnology
Laboratory
Seed counter in seed analysis
Shakers with controlled temperature and time
Other Activities:
Over all incharge for laboratory, record maintenance,
Purchase of chemicals etc
Actively involve in administration work and laboratory
maintenance
Coordination between Researchers and management
Fine tuning of the protocol and clear cut results
Other Skills:
Good experience in establishment of Biotechnology
Laboratory, Seed Technology laboratory, maintenance of
laboratory, Good coordination between research and marketing
units for conducting MAT and MLTs.
Good command on computer skills for MS Office, Page Maker,
Coral Draw, Photoshop, Internet operations, Email and
Computer application in Biotechnology field.
AREAS OF INTEREST
Technical
Invention of new techniques related to Agricultural
Research through biotechnology
Molecular Biology (Isolation of Genomic Markers)
Genetic Engineering (Gene Therapy)
Application of Biotechnology tools in Medical,
Pharmaceutical and Agricultural sectors
Non Technical
Office and General Administration
Lab Management with GLP (Good Laboratory Practices)
Project Preparation & Implementation
Team Leadership and guidance to the team as member
Targeting the deadlines with planning and implementation
without any hard ship.
Procuring the required infrastructure and chemicals for
the team as leader for easy process.
Taking overall responsibility of laboratory and reporting
to the management
Solving day to day issues of lab and team members
General
Travelling, Meeting People, discuss the latest technology
developments in Biotechnology
Learning the socio economic problems of rural public and
finding the root cause for solution.
EXPERIENCE IN DETAIL
CYGNUS
BUSINESS RESEARCH CONSULTANCY (KPO)
Designation : SENIOR CONSULTANT (BIOTECHNOLOGY)
No of Years : TWO and HALF YEAR (From October 2004 onwards
till date)
Major Assignments : 1. Consultancy in biotechnology Domine
2. Preparation of Biotechnology monitors
3. Preparation of Industry insights in biotechnology,
Bioinformatics and Pharmaceuticals and Clinical Trials
Job Profile : Technical Knowledge support for the
Biotechnology Industry in various issues like industry
outlook, Industry economy, new entries, manpower
requirement, Bioinformatics, Pharmaceutical industry with
biotechnology, Clinical Trials in Biopharma Industry for new
molecules, industry insights and industry monitors of health
care, agri biotechnology and Indian System of Medicine
BIOSEED RESEARCH INDIA LIMITED
Designation : Scientist – Biotechnology (Assistant Manager)
No of Years : 3 Years (May 2001 to August 2004)
Job Profil : Establishment of Biotechnology Laboratory (For
Genetic purity of seeds)
Standardized the Techniques for PAGE SDS Electrophoresis
Correlation and comparison of results with the
Conventional GOT
Characterization of Enzymes through Protein Bands
Preservation of Protein Gels for future reference with
photography
Nagarjuna Fertilizers and Chemicals Limited
Designation : Scientist – Biotechnology
No of Years : 6 YEARS (MARCH 1995 to MAY 2001)
Job Profile : Ø Conducting the evaluation trials for high
yield and high resistant varieties and hybrids of field
crops
Ø MLT trials for All India Coordinated trials and submitting
reports to the coordinated workshops for field crops
Ø MAT trials for market assessment
Ø Intercrop cultivation with Soybean and Cotton in farmers
field
Ø Evaluation of high yield and quality parameters of cotton
(Cotton Testing Laboratory-Incharge)
Ø Establishment of DNA Laboratory for conducting rDNA
Technology experiments
Ø Estimation of Oil percentage through NMR and viability
test for different crop seeds
Ø Germination and physical purity tests of different crop
seeds (Genetical and Physical purity)
Maharashtra Hybrid Seed Company Limited (MAHYCO)
Designation : Junior Scientist – Biotechnology
No of Years : 3 YEARS (August 1992 to January 1995)
Job Profile : Ø Establishment of Biotechnology Laboratory
Ø Extraction of Genomic DNA, RAPD and RFLP analysis, ALFP
trials
Ø Standardized techniques for rDNA Technology and
Preparation of DNA profile for ownership rights
Ø Isolation of genomic markers from the Near Isogenic lines
Ø Maintenance of instruments like PCR, UV Spectrophotomer,
Ø Electrophoresis Units, Gel Documentation, Photography
(Dark Room), Documentation of Laboratory results
Ø Handling liquids like Phenols, Liquid Nitrogen, Ethidium
bromide etc and its maintenance
Ø Purchase of required chemicals, glass ware and instruments
for biotechnology laboratory as in-charge of Laboratory.
Ø Maintenance of Restriction Enzymes, Primers and DNTPs for
proper storage, usage
STUDY DETAILS
Master of
Science M.Sc., (Applied Botany & Biotechnology)
University : Gauhati University, Gauhati, Assam State, India
Year of Pass : 1992 (1989-1991)
Thesis in M.Sc.. : Study of Sister Chromatid Exchanges in
Anesthesiologists (Medical Genetics/Biotechnology).
Bachelor of Science : Bachelor or Science B.Sc., (Botany,
Zoology & Chemistry)
University : Andhra University, (Hindu College,
Machilipatnam), AP, India.
Year of Pass : 1981 (1978-81)
PERSONAL INFORMATION
Birth
Place : Pothavaram, West Godavari Dist, Andhra Pradesh,
India.
Sex : Male
Date of Birth : 12-10-1959
Marital Status : Married
Family Members : Wife (38), Daughter (12 years)
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