|
UMA SANKAR. B
# 139, Chandralayam,
Elk hill, Near NTN,
Udhagamandalam,
The Nilgiris. 643001
Tamilnadu. INDIA
Email:
umasankar_bits@hotmail.com
Mobile: +91- 98451 06061
OBJECTIVE
To work
in a creative, exciting - research oriented setting that
expands my knowledge and provides continued focus on
organization success.
PROFESSIONAL EXPERIENCE: 4
years
Oct
2006 – Till Date
Senior Research Associate,
Sanmar Speciality Chemicals Ltd,
(Bangalore Genei R&D Div),
Bangalore. 560 058. INDIA
www.sanmargroup.com
JOB RESPONSIBILITIES
STR / Microsatellite Marker Analysis
Seed Purity Testing using Microsatellite Markers.
Development of SCAR Markers
In Vitro Mutagenesis
Gene Cloning and Expression
Molecular Diagnostics
April, 2004 – Sep 2006
Senior Research Fellow
Avestha Gengraine Technologies Pvt. Ltd,
Bangalore, INDIA www.avesthagen.com
JOB RESPONSIBILITIES
Detection of Genetically Modified Organisms using PRC
Techniques.
Seed Purity Testing using DNA Finger-Printing Techniques.
Development of Molecular Markers for Marker Assisted
Selection and Breeding
Gene Cloning, Expression, Tissue Culture & Agrobacterium
Mediated Transformation
EDUCATIONAL QUALIFICATON
Masters
Of Engineering in Biotechnology
2002 – 2003, First Class. 7.0 CGPA
BITS – Pilani, Rajasthan.
Master of Science in Biotechnology
1998 – 2000, First Class – 73.0%
Bharathidasan University, Tamilnadu.
Bachelor of Science in Biochemistry
1995 – 1998, First Class – 64.0%
Bharathiyar University, Tamilnadu.
PROJECTS
COMPLETED AT BANGALORE GENEI
Identification of basmati and non-basmati varieties using
microsatellite markers.
The objective of the project was to analyze adulteration in
basmati varieties.100 brands of basmati varieties was
procured from different parts of the country and analyzed
for non-basmati adulteration using microsatellite markers
using Genetic Analyzer tool of Applied Biosystems.
Seed Purity Testing using Microsatellite Markers.
The objective of the project was to check the Genetic purity
of cotton seeds using Microsatellite markers.
Development of a Multiplex PCR Assay for Rapid Detection
of Methicillin-Resistant Staphylococcus aureus
In this study, we developed a multiplex PCR assay, which
allows the rapid detection of S. aureus and CNS and their
associated methicillin-resistance partner. The multiplex PCR
assay developed was successful in identifying S.aureus using
Staphylococcus-genus specific primers and
methicillin-resistance strains by primers targeting the mecA
gene and primers targeting the FemB gene.
DNA fingerprinting using RAPD for almost 50 vegetable
crops.
The objective of the project was to detect polymorphism
between control and treated plants varieties of the same
lines.
Genetic relationships among ginger based on ALPL markers
The objective of the study was to understand the genetic
relationships between and within ten verities ginger of by
using AFLP Marker.
Genetic relationships among jute based on ALPL markers
The objective of the study was to understand the genetic
relationships between and within ten verities jute of by
using AFLP Marker.
PROJECTS
COMPLETED AT AVESTHAGEN
Construction of Micro-satellite Markers in Cotton for Marker
Assisted Selection and Breeding
The objective of the project was to develop 100 unique
microsatellite markers in 16 varieties of cotton for
breeding purpose. The developed microsatellite markers were
successfully tested on the16 cotton varieties provided by
the client.
Engineering secondary metabolite biosynthesis in medicinal
plants
The objective of the project was to Enhancement Of
colchicines, forskolin and other medicinally important
secondary metabolites in Gloriosa superba L. and Coleus
forskohlii.
Development of test methods and protocols to analyze DNA
product. And Quantitation of different forms of DNA in
various mixtures
The objective of the project was to develop standard
operating procedures for estimation and quantitation of
various forms of plasmid in different mixtures of
concentration.
PROJECTS COMPLETED DURING M.E., AND M.SC., PROGRAMME
Detection of Salmonella Spp using PCR Techniques at
Industrial Toxicology Research Center, Lucknow.
A 236bp DNA fragment of Hin/H2 region that is specific for
Salmonella was amplified using primers designed by Way et al
with sight modification. The method was highly specific and
sensitive for detection of Salmonella in contaminated foods.
Studies on Food Application of Beet Hairy Root and shoot
Cultures of Beeta vulgaris at Plant Cell Biotechnology
Laboratory, CFTRI, Mysore.
The pigments of red beet, Beeta vulgaris warrants
investigation as food additives. Betanine a natural pigment
is used in foods
RESEARCH INTEREST
Molecular
biology, Molecular Diagnostics, Molecular markers and its
application.
TECHNIQUES KNOWN:
Isolation and purification of Genomic DNA, Plasmids, Total
RNA and mRNA.
DNA Finger-printing (RAPD, RFLP, AFLP)
Biolistic gene Gun, Agrobacterium Mediated transformation
STR/Microsatellite markers.
Invitro Mutagenesis
PCR Techniques: RT – PCR, Long Range PCR, Multiplex PCR,
5’ & 3’ – RACE.
SDS – PAGE, 2D Gel & AGE.
Blotting Techniques: Southern and Western blotting.
Construction of Micro-satellite enriched Libraries.
Cloning and Expression.
BIOINFORMATICS AND COMPUTER
SKILLS
Operating
systems : MS-DOS, Windows 98, XP, SOLARIS
Packages : MS office
Sequence Database : Genbank, Swissprot, Prosite, Blocks.
Phylogenetic Analysis : Phylip, Treeview, Njplot.
Sequence Alignment : DNA star, Read Soft SeqScape,ClustalW,
ClustalX,
Multalin, Blast
TRAINING ATTENDED
Jan 2007
– May 2007: Application of NMR to Biological systems
Conducted by
Proficiency at Indian Institute of Science, Bangalore,
Karnataka, INDIA
16-5-2005 to 19-5-2005: Laboratory Quality Systems and
Internal Audit as per ISO/IEC 17025, Conducted by National
Accreditation Board for Testing and Calibration at Center
for Electronic Test Engineering, Bangalore, Karnataka. INDIA
6-1-1999 to 19-1-1999: Observed various procedures in
production and quality control of Rabies and DTP group of
vaccines. Pasteur Institute of India, Coonoor, Tamilnadu.
INDIA
1-7-1997 to 4-12-1997: Laboratory Technician
Vijaya Heart Hospital, Erode, Tamilnadu. INDIA
PERSONAL PROFILE:
Indian,
Single, Male.
Date of Birth: 4th Dec 1977.
Community: BC
REFERENCES
Dr.
Narinder Singh
Scientist
Avestha Gengraine Technologies Pvt.Ltd,
International Technology Park Ltd,
Whitefield Road,
Bangalore, Karnataka, INDIA.
Email:
narinder.vnarin@gmail.com
Prof. S.K. Verma,
Group leader,
Biological Science Group,
B.I.T.S – Pilani,
Rajasthan, INDIA.
Email:
skverma@bits-pilani.ac.in
Mr. Justin Jeyakani
Computational Biology Manager
Medicinal Chemistry Program
National University of Singapore
Email:
dbsjjjg@nus.edu.sg
Phone: +65 6516 1208
1840 |
back to index of job seekers |
