ASHUTOSH (Ph.D.)
R & D, Custom Services Group
Bangalore Genei (Sanmar Speciality Chemicals Ltd)
Bangalore 560058 (India)
Cell No.: 09342592690
E-mail: ashutosh.biotech@gmail.com

Job objective:

A leading and dynamic position in Biotech industry and would like to capitalize five years of doctoral R & D experience and two years of industrial experience.

Area of strength:

• Self-motivating and capable of persistent independent as well as group work, imaginative person with strong commitment to responsibilities

• Extensive hands on experience in molecular markers

• Recombinant DNA technology, transposon based sequencing, synthetic gene, Knock out construct

Educational qualifications:

Ph.D.* Bio-Technology Indian Agricultural Research Institute New Delhi & Indraprastha University Delhi -
M.Sc. Bio-Technology Dr B. R. Ambedkar University, Agra, UP 76 %
P.G. Diploma Bio-Technology Board of Technical Education Lucknow, UP 78%
(Gold medal)

* Thesis title: "Molecular characterization of cytoplasmic male sterile (Moricandia-arvensis) Brassica juncea and tagging of male fertility restorer gene"

Technical expertise:

Molecular Biology Techniques:

 Genomic DNA and RNA isolation (total and mitochondrial)
 Standard PCR, RT-PCR, RACE, rep-PCR and PCR based cloning
 Southern, Northern and western hybridization
 Transcript mapping by Primer extension technique and subtractive hybridization
 Conduct biochemical assays and enzyme assay, ELISA and immunological analysis
 Construction and screening of genomic and cDNA libraries. Gene cloning and expression, purification of protein
 Molecular markers such as AFLP, SNP, STR, RFLP, RAPD, CAPS and SCAR
 Transposon based DNA sequencing
 Construction of cloning vectors, knockout construct and synthetic gene
 In-vitro mutagenesis and develop DNA/ RNA ladders

Tissue Culture and Transformation Techniques:

 Bacterial and Plant Cultures, Induction of root, shoot, somatic embryos in various tissues ex-plants
 Protoplast isolation and culture
 Transformation of Arabidopsis and Brassica plant using Agrobacterium tumefaciens, Bacterial transformation, Bacterial conjugation
 Transfer of Ti-plasmid from E. coli to Agrobacterium by triparental mating

Bioinformatics skills:

 Comfortable with MAPMAKER, NTSYS (2.0e), Fast PCR, DNASTAR scientific software.
 Identification of novel genes from public databases, sequence-alignments and analysis of genomic sequences, knowledge of using programs and search tools like CLUSTAL W, BLAST, etc.
 Word-processing and graphic analysis using adobe photoshop, experience with scientific data and image analyzing programs.

Research Experience:

Industrial Experience:

Since February 2006 onwards, working as Research Associate in Custom Services, R & D Group, Bangalore Genei and engaged in customised project like development of molecular markers, transposon based sequencing of large DNA fragment, cloning vectors, Knockout gene construct, In-vitro mutagenesis, synthetic gene and identification of microbes based on 16S rDNA. Meeting customers and generate business are major responsibilities.

Professional Experience:

Five years (2001-2006) research experience; worked as Research Associate/Senior Research Fellow at National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.

During this period I worked on following projects…

1. Tagging of male fertility restorer gene of CMS (Moricandia arvensis) B. juncea by AFLP techniques and develop SCAR marker
2. Molecular characterization of CMS lines of Brassica juncea
3. Cloning and characterization of cytoplasmic male sterility associated transcript of Brassica juncea

Positions held:

1. Senior Research Associate,
Custom Services Group, R&D
March, 2008 to present
Bangalore Genei, Sanmar Speciality Chemicals Ltd.
Bangalore, India


2. Research Associate,
Custom Services Group, R&D
Feb., 2006 to Feb, 2008
Bangalore Genei, Sanmar Speciality Chemicals Ltd.
Bangalore, India

Job responsibilities:

• Meeting customer, deliver presentations and develop business
• Literature survey, writing SOP manuals and project reports in ISO format and guidelines, development of new protocol are also part of my job responsibilities
• Qualified internal ISO auditor
• Working on multiple projects:
# Transposon based sequencing of large DNA fragment
# Development of molecular markers in plants, allele sizing, genotyping.
# Construct cloning vector and develop knockout construct
# Synthetic gene, codon optimization and in-vitro mutation

3. Research Associate,
Sept. 2002 to Jan. 2006
National Research Center on Plant Biotechnology,
Indian Agricultural Research Institute
New Delhi 110012

4. Senior Research Fellow,
Feb, 2001 to Sept. 2002.
National Research Center on Plant Biotechnology,
Indian Agricultural Research Institute
New Delhi 110012

Scholarships/Awards/Fellowships:

Only Indian who received the International Travel grant from Department of Biotechnology, Govt of India to present research work in The Annual meetings of Plant Biologists "Plant Biology-2005" held at Seattle, Washington, U.S.A. (July 16-20, 2005).

Awarded Research Associateship by Indian Council of Agricultural Research (ICAR), New Delhi, India (September 2002 onwards)

Qualified Agricultural Research Service (ARS) National Eligibility Test 2005 for Lectureship (Plant Biotechnology)

Awarded Senior Research Fellowship by National Agricultural Technology Program (NATP), India (Feb. 2001 - Sept. 2002)

Qualified Junior Research Fellowship /Lectureship National Eligibility Test (NET) 2002 & 2003 conducted by Council for Scientific and Industrial Research, New Delhi, India

Qualified Graduate Aptitude Test in Engineering (GATE) 2001 conducted by Indian Institute of Technology (IIT), Kanpur, India

Awarded Gold Medal in P.G. Diploma Biotechnology (1998)

Symposium/Workshops/Conferences attended:

1. The Annual Meetings of American Society of Plant Biologists 'Plant Biology 2005' Seattle, Washington, USA, 16-20 July, 2005.

2. International Conference on Resource Development and Marketing Issues in
Rapeseed-Mustard, Jaipur, India 28-29 March, 2005.

3. Indraprastha Regional Workshop on Intellectual Property Rights, Guru Govind Singh Indraprastha University, Delhi, India 29-30 October, 2004.

Publications: (total impact factor ~12)

Manuscripts:

1. Ashutosh, Pankaj Kumar, P. C. Sharma, Shyam Prakash, S. R. Bhat (2008) A novel orf108 co-transcribed with atp A gene is associated with cytoplasmic male sterility in Brassica juncea carrying Moricandia arvensis cytoplasm. Plant Cell and Physiology 49: 284-289.

2. Ashutosh, P. C. Sharma, Shyam Prakash, S. R. Bhat (2007) Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia-arvensis) Brassica juncea and conversion to SCAR marker. Theortical and Applied Genetics 114: 385-392.

3. S. R. Bhat, PriyaVijayan, Ashutosh, K. K. Dwivedi, and S. Prakash (2006) Diplotaxis erucoides-induced cytoplasmic male sterility in Brassica juncea is rescued by Moricandia arvensis restorer: Genetic and molecular analyses. Plant Breeding 125:150-155.

4. A. Pathania, Rajesh Kumar, V.Dinesh Kumar, Ashutosh, K.K. Dwivedi, P.B. Kirti, S.Prakesh, V.L.Chopra and S.R.Bhat, (2006) A duplication of Cox I gene is associated with CMS(Diplotaxis catholica) Brassica juncea derived from somatic hybridization with Diplotaxis catholica. Journal of Genetics 86:93-101.

5. Ashutosh, K. K. Dwivedi, V. Dinesh Kumar, Shyam Prakash and S. R. Bhat, (2005) rep-PCR helps to distinguish different alloplasmic cytoplasmic male sterile lines of Brassica juncea Plant Science 168(4): 1083-1087.

6. A. Pathania, S. R. Bhat, V. Dinesh Kumar, Ashutosh, P. B. Kirti, S. Prakash and V.L. Chopra, (2003) Cytoplasmic male sterility in alloplasmic Brassica juncea carrying Diplotaxis catholica cytoplasm: molecular characterization and genetics of fertility restoration. Theortical and Applied Genetics 107: 455-461.

Posters/ Abstracts:

1. Ashutosh, K. K. Dwivedi, P. C. Sharma, Shyam Prakash, S. R. Bhat (2005) Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia-arvensis) Brassica juncea and conversion to SCAR marker. The Annual Meetings of American Society of Plant Biologists 'Plant Biology 2005' Seattle, Washington, U.S.A.16-20 July 2005, pp 311.

2. Ashutosh, K. K. Dwivedi, S. R. Bhat, P. C.Sharma, Shyam Prakash, (2005) Molecular tagging of a unique male fertility restorer gene of CMS (Moricandiaarvensis) Brassica juncea. International Conference on Resource Development and Marketing Issues in Rapeseed-Mustard, Jaipur, India March 28-29, 2005, pp 51.

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