New York, New York
July 21, 2003
By a
GenomeWeb staff reporter
The Maize Mapping Project -- a collaboration between the
University of Missouri-Columbia, the University of Arizona
Genomics Institute, and the University of Georgia -- has
completed the first phase of an integrated genetic and physical
map for the corn genome.
The project, which is anchoring ESTs and other markers to both
the physical and genetic maps of the maize genome, recently
concluded the first of three scheduled phases, said Ed Coe, a
University of Missouri geneticist and principal investigator on
the project. Phase I entailed both computational assembly of BAC
contigs as well as a manual curation step that reduced the total
number of contigs from around 300,000 to a "workable number" in
the neighborhood of 3,000, Coe told GenomeWeb.
The maize genome has approximately 2.5 billion base pairs
arranged into 10 chromosomes. "Half of the total DNA [in the
maize genome] is pinned down now," said Coe.
The five-year mapping project is scheduled to end in September.
Phase II will entail further manual editing of the genetic and
physical maps, and Phase III will use comparative genomics to
gain further knowledge about the maize genome by comparing it to
the genomes or rice and sorghum, Coe said.
"This is the most exciting phase for our project because we're
seeing it all come together after a period of slow, step-wise
progress," said Coe.
The BAC contigs and genetic/physical anchoring are available at
two sites:
www.genome.arizona.edu/fpc/maize/ and
www.maizemap.org/iMapDB/iMap.html.
The physical and genetic maps will provide the groundwork for
large-scale maize genome sequencing projects. The NSF began
awarding maize sequencing grants in the fall as part of a broad
plant genomics initiative: A two-year project led by Rutgers
University received a $4.3 million NSF grant to sequence 20
million base pairs of the maize genome; while a project led by
the Donald Danforth Center in St. Louis was awarded $6 million
for a two-year project focusing on sequencing gene-rich regions
of the maize genome. |