Palo Alto, California
August 4, 2004
Agilent Technologies Inc. (NYSE: A) today announced it has
introduced the first whole-genome oligonucleotide microarray for
the study of Arabidopsis thaliana, the primary model plant used
in gene expression research. By revealing gene activity
associated with various biological functions, stages of growth
and response to stress in Arabidopsis, the Arabidopsis 3 oligo
microarray kit allows scientists to understand these processes
in common crop plants such as corn, soybean and cotton.
Scientists will use this knowledge to improve crop yields;
enhance the quality of plant fibers, foods and other materials;
and increase plant resistance to disease and drought.
Arabidopsis, a noncommercial member of the mustard family, has
become one of the most popular models in plant research because
its life cycle and response to stress and disease resemble those
of many crop plants. It is easy to grow and widely available,
which facilitates large-scale experiments. The plant also has a
relatively small genome that has been completely sequenced,
making it useful in genetic analyses(1).
Agilent's new microarray contains more than 37,000 probes,
representing all Arabidopsis genes of known function and related
genes of interest. The content includes probes for approximately
26,000 annotated transcripts from the ATH1v5 database of The
Institute for Genomic Research (TIGR). Annotations are
descriptions of gene function linking gene expression patterns
with the biology of the plant, and are critical to interpreting
the results of gene expression experiments. The microarray also
covers more than 10,000 unannotated transcripts from the
Massively Parallel Signature Sequencing (MPSS) database of the
University of Delaware and 1,000 ncRNA (small non-protein
coding) transcripts derived from the ATH1 genome.
"Our new Arabidopsis microarray provides a major leap in content
coverage over current competitive products," said Mel Kronick,
chief scientist of Agilent's Integrated Biology Solutions unit.
"In addition to covering all known protein coding transcripts of
the Arabidopsis genome, it is also the first to provide probes
for unannotated genomic regions, which include suspected
transcription control factors of great interest to researchers
studying plant transcriptional processes."
Agilent uses a flexible inkjet manufacturing process to
synthesize its probes directly on glass slides to a length of 60
oligonucleotides. This process produces microarrays with the
highest sensitivity available, five to eight times more
sensitive than 25 mer oligo microarrays, allowing for better
detection of low-expressing or rare genes. It also enables
Agilent to iterate its microarray design files quickly to keep
pace with the latest changes in genome content.
Based on the industry-standard 1" x 3" glass-slide format,
Agilent's whole genome oligo microarrays can be read on most
commercial microarray scanners. Agilent's open-platform enables
scientists to easily migrate from their lab-made "home brew"
microarrays to high-quality commercial microarrays with the
lowest startup costs.
Researchers can take best advantage of the increased genome
content coverage and high sensitivity by using Agilent's
high-performance DNA microarray scanner and feature extraction
software. Agilent also provides a complete line of labeling and
hybridization reagents, chambers and accessories that maximize
the ease of use and performance of Agilent microarrays.
Agilent Technologies Inc. (NYSE: A) is a global technology
leader in communications, electronics, life sciences and
chemical analysis. The company's 28,000 employees serve
customers in more than 110 countries. Agilent had net revenue of
$6.1 billion in fiscal year 2003. |