Date of publication: March 4, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/NL/03/09
Member State:Netherlands
Date of Acknowledgement:10/11/2003
Title of the Project: Application for the release into
the environment of potato event EH92-527-1
Proposed period of release From:01/04/2004
To:31/12/2008
Name of the Institute(s) or Company(ies): BASF Plant
Science GmbH;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Germany; Sweden
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s): -
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| potato
|
solanaceae
|
solanum
|
solanum
tuberosum |
tuberosum
|
Prevalent
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
A gene modifying the starch composition in potato tubers
raising the level of amylopectin has been introduced into
potato. The neomycin phosphotransferase II gene has been used as
selectable marker gene.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
In potato event EH92-527-1 a gene consisting of a potato gbss
(granule bound starch synthase) promoter, a fragment of the
coding region of potato gbss in antisense orientation relative
to the promoter and the polyadenylation sequence from
Agrobacterium tumefaciens nopaline synthase gene (gene construct
pHoxwG) have been inserted into potato variety Prevalent thus
reducing the amount of amylose in the starch fraction. In
addition the neomycin phosphotransferase gene (nptII) connected
to the Agrobacterium tumefaciens nopaline synthase promoter and
polyadenylation signal has been inserted as selectable marker
gene conferring resistance to kanamycin.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
The release is foreseen as part of the application process
for variety registration and variety protection of potato event
EH92-527-1.
2. Geographical location of the site:
The location of the release site will be at Borger-Odoorn,
The Netherlands.
3. Size of the site (m2):
1 ha in 2004.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Comprehensive data from previous releases have shown that
potato clone EH92-527-1 is compositionally - with the exception
of the modified starch structure - as well agronomically
equivalent to the parent variety Prevalent. There is no evidence
that insects associated with the GM clone are more or less
abundant compared with the non-GM parent. The GM clone is no
more susceptible or resistant to aphids, leafhoppers, larvae,
worms, snails or potato cyst nematodes than the parent variety.
Similarly there is no evidence for modified susceptibility to
Phytophthora, Erwinia, Alterniaria and the viruses PVP, PLRV,
PMTV and TRV. No adverse impacts on the environment and human
health were observed during any field trials carried out so far.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato line shows a shift of the
tuber starch composition to amylopectin. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM lines via the introduced traits.
Interactions of the GM potato lines with non-target species and
resulting effects will be comparable to those of conventional
potato varieties. No toxic or allergenic effects are expected
from elevated levels of amylopectin starch, nor from the
neomycin phosphotransferase. Measures in place under current
field trial practice will protect the trial from damage by
animals as well as safeguard that all seed and plant material is
properly managed, harvested, stored, transported or disposed of
to minimize or prevent contact to humans or animals. No effects
are expected on biogeochemical processes. The GM potato lines
will be cultivated under conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
Planting and harvesting equipment will be cleaned on site to
prevent the dispersal of GM tubers. There will be no potato
cultivation on the release area for the year following the
release. Potential volunteers will be monitored and removed
according to conventional agricultural practice. During the
release the trial site will be monitored at defined intervals.
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