Deliberate release into the
environment of GMOs for any other purposes than placing on the
market
Application for the release into the
environment of potato events AM02-1003, AM02-1005, AM02-1012,
AM02-1017 and AM99-1089 - BASF Plant Science GmbH |
Date of publication: March 4, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/NL/03/10
Member State: Netherlands
Date of Acknowledgement:10/11/2003
Title of the Project:
Application for the release into the environment of potato
events AM02-1003, AM02-1005, AM02-1012, AM02-1017 and AM99-1089.
Proposed period of release From:01/04/2004
To:31/12/2008
Name of the Institute(s) or Company(ies): BASF Plant
Science GmbH;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Germany; Sweden
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s): -
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| potato
|
solanaceae
|
solanum
|
solanum
tuberosum |
tuberosum
|
Seresta,
Kuras |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
A gene modifying the starch composition in potato tubers
raising the level of amylopectin has been introduced into
potato. The neomycin phosphotransferase II gene or an
acetohydroxyacid synthase gene have been used as selectable
marker genes.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
a) A gene containing the coding region of potato gbss in
antisense orientation relative to promoter, flanked by the gbss
promoter from Solanum tuberosum and the polyadenylation sequence
from Agrobacterium tumefaciens nopaline synthase gene has been
inserted into potato variety Seresta (lines AM02-1003,
AM01-1005, AM02-1012) and Kuras (line AM02-1017) thus reducing
the amount of amylose in the starch fraction. An ahas gene
(acetohydroxyacid synthase) from Arabidopsis thaliana flanked by
the Agrobacterium tumefaciens nos gene promoter and the octopine
synthase polyadenylation sequence serves as selectable marker
gene conferring tolerance to Imazamox.
b) AM99-1089 serves as a reference line. The inserted gene
consists of the potato gbss (granule bound starch synthase)
promoter, the coding region of potato gbss in antisense
orientation and the polyadenylation sequence from Agrobacterium
tumefaciens nopaline synthase gene thus reducing the amount of
amylose in the starch fraction. In addition the neomycin
phosphotransferase gene (nptII) connected to the Agrobacterium
tumefaciens nopaline synthase promoter and g7 polyadenylation
sequence from Agrobacterium tumefaciens has been inserted as
selectable marker gene conferring resistance to kanamycin.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
Experimental
Release
1. Purpose of the release:
The purpose of the release is, within the frame of safety
studies for events AM02-1003, AM02-1005, AM02-1012, AM02-1017
and AM99-1089 (as a comparator high amylopectin event), to
compile data on agronomical performance and environmental
effects, as well as to collect plant material for further
analyses (e.g. biochemistry, molecular biology). It is
anticipated that in part the trial will be used as well to
generate seed tubers.
2. Geographical location of the site:
The location of the release site will be at Borger-Odoorn,
The Netherlands.
3. Size of the site (m2):
1 ha.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for the lines AM02-1003, AM02-1005, AM02-1012,
AM02-1017 and AM99-1089 in previous field trials in Sweden.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines show a shift of the
tuber starch composition to amylopectin. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM lines via the introduced traits. Due to the
measures to be taken during the release (distance to or absence
of cultivated and wild relatives) will the potential for gene
transfer during the release excluded. Interactions of the GM
potato lines with non-target species and resulting effects will
be comparable to those of conventional potato varieties. No
toxic or allergenic effects are expected from elevated levels of
amylopectin starch, nor from the neomycin phosphotransferase or
acetohydroxyacid synthase. Measures in place under current field
trial practice will protect the trial from damage by animals as
well as safeguard that all seed and plant material is properly
managed, harvested, stored, transported or disposed of to
minimise or prevent contact to humans or animals. No effects are
expected on biogoechemical processes. The GM lines will be
cultivated under conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
An isolation distance of 20 m to other potato varieties will
be observed. Planting and harvesting equipment will be cleaned
on site to prevent the dispersal of GM tubers. There will be no
potato cultivation on the release area the year following the
release. Potential volunteers will be monitored and removed
according to conventional agricultural practice. During the
release the trial site will be monitored at defined intervals. |
|
|
|
