Australia
November 17, 2005
Research by
CSIRO to genetically modify
peas to resist insect attack and reduce the use of chemical
sprays has been discontinued because the GM peas did not satisfy
all categories of a stringent risk assessment process.
The Deputy
Chief of CSIRO Plant Industry, Dr TJ Higgins, says the findings
– published this week in the
Journal of
Agricultural and Food Chemistry – demonstrate the
effectiveness of case-by-case evaluation of GM plants and the
important role science can play in decision-making around the
introduction of GM crops.
The GM field
peas were developed by CSIRO Plant Industry to protect
Australia's $100 million field pea industry from the pea weevil
Bruchus pisorum, which can cause yield losses of up to 30
per cent each year if left uncontrolled.
Although
this GM breed of field pea proved almost 100 per cent effective
against pea weevil attacks, research led by immunologists Dr
Simon Hogan and Professor Paul Foster at the John Curtin School
of Medical Research (JCSMR), with CSIRO, showed that the GM peas
caused an immune response in mice.
Following
discussions with the scientists conducting the study, CSIRO
decided not to progress development of these GM field peas.
“This work
strongly supports the need for case-by-case examination of
plants developed using genetic modification and the importance
of decision-making based on good science,” Dr Higgins says.
“Even
though this GM field pea research will not be progressed
further, the technology is very valuable and we're considering
applying it to other research,” he says.
The CSIRO
research team used a gene from beans to block the activity of
alpha-amylase, an enzyme important for digestion of starch.
Weevil
larvae feeding on starch in the developing pea seed are unable
to digest the starch and starve.
“We asked
why there was a reaction to the GM peas and not beans, which
also have the alpha-amylase inhibitor, and which humans have
been eating for many years without evidence of an immune
response,” JCSMR's Professor Foster says.
The answer
lay in subtle changes that occurred in the chemical structure of
the bean alpha-amylase inhibitor when it was made in the field
pea.
“The change
in structure is likely to be caused by a natural and commonly
occurring process called glycosylation, which occurs when
proteins are made via a particular pathway in cells,” Professor
Foster says.
“CSIRO had
informed us that unlike other GM insect resistant plants, to
make the peas insect resistant the introduced bean protein had
to go through a pathway in cells where it would undergo several
processing steps including glycosylation.
“Because
glycosylation is well documented and because it can differ from
organism to organism and even in different cell types within an
organism, we determined the structure of the pea protein, and
assessed whether it was likely to cause an immune response.
“This
case-by-case approach allows appropriate decisions to be made
during the development of the GMO, and CSIRO has done this with
its GM field peas.”
CSIRO is
finalising arrangements with the Office of the Gene Technology
Regulator for the disposal of GM field peas produced during the
project.
1. Fact
sheet at
www.csiro.au/GMpeas
2.
Transgenic Expression of Bean á-Amylase Inhibitor in Peas
Results in Altered Structure and Immunogenicity
Vanessa E. Prescott, Peter M. Campbell, Andrew Moore, Joerg
Mattes, Marc E. Rothenberg, Paul S. Foster, T. J. V. Higgins,
and Simon P. Hogan
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume 53, Issue 23
(November 16, 2005)
pages 9023 - 9030 |