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October 18, 2005
Plant
Biotechnology Journal
Volume 3 Issue 6 Page 555 - November
2005
High-lysine corn produced by
the combination of enhanced lysine biosynthesis and reduced zein
accumulation
Shihshieh Huang,
Diane E. Kruger, Alessandra Frizzi, Robert L. D'Ordine, Cheryl
A. Florida, Whitney R. Adams, Wayne E. Brown and Michael H.
LuethySUMMARY
Corn is one of the major crops
in the world, but its low lysine content is often problematic
for animal consumption. While exogenous lysine supplementation
is still the most common solution for today's feed corn,
high-lysine corn has been developed through genetic research and
biotechnology. Reducing the lysine-poor seed storage proteins,
zeins, or expressing a deregulated lysine biosynthetic enzyme,
CordapA, has shown increased total lysine or free lysine content
in the grains of modified corn plants, respectively. Here, by
combining these two approaches through genetic crosses, the
total lysine content has more than doubled in F1 progeny. We
also observe a synergy between the transgenic zein reduction and
the enhanced lysine biosynthesis by CordapA expression. The zein
reduction plants are found to accumulate higher levels of
aspartate, asparagine and glutamate, and therefore, provide
excess precursors for the enhanced lysine biosynthesis.
Plant
Biotechnology Journal subscribers can access the full
article at
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1467-7652.2005.00146.x
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