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Barley gene find could mean more beer per bushel

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Bundoora, Victoria, Australia
April 17, 2007

Beer lovers and barley growers could soon be raising their glasses to Australian scientists who may have found a way to increase beer supply.

Researchers at the Molecular Plant Breeding CRC are looking for genes that protect barley from pre-harvest sprouting, a grain condition that causes substantial reductions in barley yield and significant economic losses for farmers.

Under prolonged wet or damp conditions, barley grains begin to germinate or ‘sprout’. Sometimes this happens while the crop is still standing, before the barley is harvested.

Pre-harvest sprouted barley is useless for beer making because the starch has been degraded and the grain can’t be malted.

Instead, farmers are forced to sell their grain in the considerably less profitable animal feed market, and beer drinkers are denied the ales and lagers for which Australian barley is known.

Ms Yumiko Bonnardeaux, a PhD student at the WA Department of Agriculture and Food, says that pre-harvest sprouting could be eradicated by breeding barley lines that contain ‘dormancy genes’.

Similar to the way bears hibernate in the winter and emerge in spring when the weather is warmer and food plentiful, the seeds of many plants have a mechanism which enables them to only germinate when the conditions are favourable for growth.

“Warm temperatures or rain can act as cues for seeds, telling them that the time is right for germination,” says Ms Bonnardeaux.

“But dormant seeds are prevented from germinating, even under the conditions that encourage germination.”

“The greater number of dormancy genes a barley plant has, the greater its resistance to pre-harvest sprouting.”

“The challenge is to develop barley grain that is dormant enough to withstand pre-harvest sprouting but not so deeply dormant that the grain doesn’t germinate during the malting.”

Ironically, Ms Bonnardeaux says, pre-harvest sprouting is a result of our own quest for the perfect beer.

“Over the years, farmers have continually selected and bred varieties with lower seed dormancy to provide grain that will germinate rapidly in the malthouse. As a result, malting varieties often have low dormancy and are more susceptible to preharvest sprouting.”

Ms Bonnardeaux has found pre-harvest sprouting to be a very complicated trait controlled by multiple genes and environmental factors.

“Depending on the environment, different genes controlling dormancy get switched on. That’s why grain from the same barley variety may have a different dormancy response depending on the site that it’s grown on.”

“This is also why it’s difficult for breeders to reliably predict which barley lines will have pre-harvest sprouting tolerance, as this can change from year to year.”

As a part of her PhD research, Ms Bonnardeaux has located regions of barley chromosomes that may contain previously unidentified dormancy genes. These have been tested extensively across different sites and over two seasons for pre-harvest sprouting.

Using the well studied plant Arabidopsis, or ‘Thale cress’, as a guide, she has identified several potential dormancy genes and is now mapping these in the same barley population.

“The next step is to find out whether these genes are located in chromosomal regions known to be involved in controlling dormancy in barley. This would identify whether these are candidate genes to do further research on.”

Once it is certain which genes confer the dormancy ability, molecular tests can be developed that predict which varieties have these genes. Plant breeders will then be able to develop varieties that are tolerant to pre-harvest sprouting.

The Molecular Plant Breeding CRC is a Cooperative Research Centre established under the Australian Government's CRC Program.

 

 

 

 

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