The project is collaboration between an academic laboratory (University of Orléans) and a seed company (Limagrain Europe). The objective is to study DNA methylation in various maize accessions. The global DNA methylation using HPLC method as well as microarrays will be done. This last technology allows the identification of the methylation state of thousands sequences in one experiment and could be performed in parallel for several biological samples.
The candidate (with a doctorate in the field of biology) will have a perfect knowledge of microarray technology and in particular the biocomputing analyses, the associated databases and statistics. Skills in molecular biology would be appreciated as well as some knowledge of the HPLC technique. The candidate will collaborate with two teams (Limagrain and LBLGC) on 2 sites (Orléans and Clermont-Ferrand) and will need a strong autonomy for the management of the project and the preparation of reports and publications (fluent English). The candidate will be located in Orléans but will have to move in both cities of the project (several stays of one week financially integrated into the project). The candidate will be available before summer, 2012 and for at least one year.
This email will include a detailed CV and a letter in support of application underlining your adequacy with the wanted profile.
The recruiters :
LIMAGRAIN EUROPE : We are in Europe the “Field Seeds” branch of LIMAGRAIN GROUP, agriculture and food, international and innovative Group, bringing together 2000 farmer members. The Group has 7200 employees of 45 nationalities in 38 countries. Its turnover is 1.5 billion euros.
LIMAGRAIN EUROPE (1250 permanent employees) is part of the Group’s values and is, together with its subsidiaries, among the leaders. From Research to Commercialization, our control of all process allows us to offer our customers high quality products that meet the needs and market developments (B to B). We sell our products in over 50 countries through our subsidiaries and our distribution partnerships.
The genotyping laboratory located in Chappes has for objective to assist the Limagrain breeders working on field crop species such as maize, wheat, barley and rapeseed. The unity of development is positioned upstream to the genotyping activity and is responsible in particular for the implementation of new protocols and technologies for the routine activity of the laboratory.
UNIVERSITY OF ORLEANS - LBLGC : The Laboratory of woody and crop plants (laboratoire de Biologie des Ligneux et des Grandes Cultures; LBLGC - EA 1207) is composed of 40 persons: 5 professors, 16 assistant-professors, 11 ingenior/techncians and 8 PhD-students/Postdoc. The laboratory is organized in three teams on two sites: the campus of the University of Orléans and the campus of Chartres. The team ‘ARCHE’ in Orléans is also a unit of INRA the National Agronomy Research Institute (USC1328 Director Pr. F. Brignolas). Our team works on poplar × water availability and focus particularly on two traits, the water use efficiency and the drought tolerance. One axis of this research is studying the role of epigenetic mechanisms (Dr Stéphane Maury). We have previously shown the role of DNA methylation in morphogenesis (Causevic et al., 2005; Causevic et al., 2006; Teyssier et al., 2008) but also in response to environmental trigerring (Gourcilleau et al., 2010; Trap-Gentil et al., 2011). In addition, the DNA methylation polymorphism in plants is a source of potential biomarkers for molecular assisted selection in breeding (Gentil and Maury, 2007; Maury et al., in press).
More positions from: Limagrain Europe
Posted from April 19, 2012 until October 19, 2012
SeedQuest reference number: 26250