Ebersberg, Germany
February 14, 2011

Using different types of genomic DNA libraries enables sequencing and scaffolding of genomes of any size

Eurofins MWG Operon, an expert in next generation sequencing (NGS) routinely uses up to 5 different non-cloned genomic DNA libraries for sequencing with Roche GS FLX or Illumina HiSeq 2000 technology. The libraries are a combination of shotgun libraries and long paired end (LPE) libraries with different jumping distances (3 kb, 8 kb, 20 kb and up to 40 kb). After sequencing, the resulting reads are assembled into large contigs and merged to scaffolds. Additional sequencing of normalised or methyl depleted libraries allows deep sequencing of euchromatic regions in highly repetitive genomes.

“Today, the multiple library approach of Eurofins MWG Operon is the most efficient and most economic way to sequence and scaffold genomes”, says Prof Ken Wolfe, The Smurfit Institute of Trinity College, Dublin, Ireland. “I was impressed by the excellent quality of the assembly and the fast progress of the project.

“One of the biggest challenges in next generation sequencing is the assembly and scaffolding of the huge amount of sequence data”, says Bruno Poddevin, Eurofins Senior Vice President, Genomic Services. “I am extremely pleased about the performance and unique advantages of the LPE libraries which have been evolved by our NGS development team.
 

Website: http://www.eurofins.com

Published: February 14, 2011