Gilroy, California, USA
Source: Eurofins STA eNewsletter
Our colleagues at Bioreba AG announced the development (in cooperation with the Faculty of Agricultural and Food Sciences, American University of Beirut, Lebanon) of ELISA Reagents with specificity to Cucurbit yellow stunt disorder virus.
Cucurbit yellow stunting disorder virus (CYSDV- Closteroviridae, genus Crinivirus) causes serious damage to cucurbit crops with an approximated yield reduction of 30-50%. Different species of cucurbits (i.e., melons, cucumber, squash and watermelon) are affected, but other hosts such as lettuce, alfalfa, bean and weed species could experimentally be infected as well. Symptoms normally appear as bright yellowing of older leaves. CYSDV symptoms are often confused with nutrient deficiency symptoms.
The phloem-limited virus is neither seed nor mechanically transmitted, but is transmitted with infected propagation material (transplants) and by the whitefly vector Bemisia tabaci. The disorder occurs mainly in areas where the whitefly has been established, e.g., the Arabic Peninsula, Eastern and Western Mediterranean basin, some African countries and North America and was first discovered in the United Arab Emirates
Specificity and sampling instructions
These DAS-ELISA reagents contain polyclonal antibodies made against a recombinant coat protein of CYSDV; the original viral RNA was isolated from infected, greenhouse-grown cucumbers in Lebanon. The antibodies have been validated using infected and healthy plants of different cucurbit species (such as cucumber and melon); they are suitable for specifically recognizing CYSDV from cucurbits.
The virus can be detected in leaves throughout the whole growing season. The highest virus concentration is present in leaf tissues (leaf mid vein and petioles) showing early symptoms of interveinal chlorosis.
For ordering, prices, and further information please contact Donna Arguello (1- 800-426-9124 or email@example.com).