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Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew


China
July 2014

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

Yanpeng Wang,Xi Cheng,Qiwei Shan,Yi Zhang,Jinxing Liu,Caixia Gao,& Jin-Long Qiu

Nature Biotechnology
DOI:10.1038/nbt.2969

Abstract

Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes1, but simultaneous modification of multiple homoeoalleles has not been reported. Here we use transcription activator–like effector nuclease (TALEN)2, 3 and clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (refs. 4,5) technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEW-RESISTANCE LOCUS (MLO) proteins6. Genetic redundancy has prevented evaluation of whether mutation of all three MLO alleles in bread wheat might confer resistance to powdery mildew, a trait not found in natural populations7. We show that TALEN-induced mutation of all three TaMLOhomoeologs in the same plant confers heritable broad-spectrum resistance to powdery mildew. We further use CRISPR-Cas9 technology to generate transgenic wheat plants that carry mutations in the TaMLO-A1 allele. We also demonstrate the feasibility of engineering targeted DNA insertion in bread wheat through nonhomologous end joining of the double-strand breaks caused by TALENs. Our findings provide a methodological framework to improve polyploid crops.



More solutions from:
    . Chinese Academy of Sciences
    . Institute of Genetics and Developmental Biology


Website: http://www.cas.cn

Published: July 22, 2014


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