Date of publication: March 18,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/ES/05/15
Member State: Spain
Date of Acknowledgement:11/02/2005
Title of the Project:
Testing of genetically modified maize resistant to certain
Coleopteran insects and tolerant to herbicides
Proposed period of release From:01/04/2005
To:31/12/2008
Name of the Institute(s) or Company(ies): PIONEER
HI-BRED SPAIN S.L.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
experimental varieties |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The genetically modified 59122xNK603 maize varieties that
will be tested in these field trials are resistant to certain
Coleopteran insects, such as the Western corn rootworm
(Diabrotica virgifera virgifera) and can tolerate the use of
glufosinate-ammonium and glyphosate herbicides for the weeding
of maize field. This maize was derived through traditional
breeding methods between progeny of genetically modified 59122
maize, which is resistant to certain coleopteran insects and
tolerant to glufosinate herbicide, and NK603 maize (from
Monsanto), which is tolerant to glyphosate herbicide.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
59122xNK603 maize contains the following genetic elements:
- the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis
strain PS149B1 that confer resistance to certain Coleopteran
insects, such as Diabrotica virgifera virgifera, and the pat
gene from Streptomyces viridochromogenes that confers tolerance
to glufosinate-ammonium herbicide, from 59122 maize
- the cp4epsps genes from Agrobacterium sp. strain CP4 that
confer tolerance to glyphosate herbicide, from NK603 maize
All these genes have been introduced with only their regulatory
components necessary for expression.
6. Brief description of the method used for the genetic
modification:
No genetic modification was made on the 59122xNK603 maize
varieties directly: they were derived through traditional
breeding methods between progeny of the genetically modified
59122 maize and NK603 maize.
59122 maize was genetically modified using Agrobacterium
transformation method. NK603 maize was genetically modified by
Monsanto using the particle acceleration method.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
Several types of trials are planned, to collect various maize
tissue samples for protein expression analysis, to control maize
volunteers with different herbicides, to evaluate the residual
behavior of glufosinate and glyphosate in transgenic maize, to
collect data on the agronomic value and insect resistance of
transgenic maize varieties
2. Geographical location of the site:
The release is planned at the following locations in Spain in
2005:
- Aragón: Pastriz (Zaragoza), Gurrea de Gállego (Huesca).
- Cataluña: Alpicat (Lérida), Alguaire (Lérida).
- Madrid: Estremera (Madrid).
- Navarra: Fustiñana (Navarra)
- Castilla-La Mancha: Membrilla (Ciudad Real)
3. Size of the site (m2):
Each site in 2005 could have up to 600 m2 sown with the
genetically modified maize concerned by this application
(59122xNK603 maize), the total area taken up by the release (all
varieties and borders included) being higher.
In the following years, there could have up to 5000 m² of the
genetically modified maize subject of this notification sown per
site, the total trial surface at each site (all varieties and
borders included) being larger.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Field trials have already been conducted at several locations
across maize growing regions in North America, South America and
Europe and no environmental problems were reported for these
trials. The transgenic plants were indistinguishable from the
non-genetically modified maize plants except for showing
resistance to certain Coleopteran insects such as the Western
corn rootworm and showing tolerance when glufosinate and
glyphosate herbicides have been applied, traits due to the
genetic modifications.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from
the deliberate release of genetically modified maize resistant
to certain Coleopteran insects and tolerant to glufosinate and
glyphosate herbicides are expected, as per information contained
in the environmental risk assessment included in the
notification.
Brief description of any measures taken for the management of
risks:
The shedding of pollen from the genetically modified plants
will be controlled by maintaining a 200-meter isolation distance
with any other non-experimental maize crop. In addition, the
trial site will be surrounded by four border rows of
conventional maize of a similar relative maturity that will be
also destroyed at the end of the release.
Seed dispersal of individual kernels does not generally occur.
They are fixed on a cob and enclosed in many husks that protect
the seeds from outside contact. When a few seeds need to be
collected for analysis; this will be done by sampling the whole
ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by
application of a non-selective herbicide, other than glufosinate
and glyphosate, or by mechanical destruction, and incorporation
into the soil.
At the end of the release, all remaining plant matter that has
not been harvested for analyses will be destroyed by chopping
and incorporating into the soil.
Volunteer maize will be monitored during the following year. An
herbicide treatment (other than glufosinate and glyphosate) will
be used in order to ensure destruction if needed.
There will be no commercial maize grown on the same field the
following year.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable to this release. |
