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Deliberate release into the E.U. environment of GMOs for any other purposes than placing on the market:
Notification for the deliberate release of Oilseed Rape lines genetically modified for increased and stable grain yield
Date of publication: February 14, 2006
Source: http://gmoinfo.jrc.it/gmp_browse_geninf.asp

Notification number:  B/SE/06/724

Member State:Sweden

Date of Acknowledgement:01/02/2006

Title of the Project:
Notification for the deliberate release of Oilseed Rape lines genetically modified for increased and stable grain yield

Proposed period of release From:01/01/2006 To:31/12/2010

Name of the Institute(s) or Company(ies): Plant Science Sweden AB;

3. Is the same GMPt release planned elsewhere in the Community?
Not known

4 - Has the same GMPt been notified elsewhere by the same notifier?
Yes
If yes, notification number(s):
-

Other notifications
05-BAS1-037-CAN01-0983-MB004-01; 05-BAS1-037-CAN02-0984-MB004-01; 05-BAS1-037-CAN03-0985-MB004-01

Genetically modified plant

1. Complete name of the recipient or parental plant(s)
Common Name Family Name Genus Species Subspecies Cultivar/breeding line
spring oilseed rape brassicaceae brassica brassica napus napus (syn. oleifera) Westar

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- improved and stabilized grain yield in spring oilseed rape under a range of environment conditions
- neomycin phosphotransferase II gene as selectable marker gene to identify transgenic cells in tissue culture

Genetic modification

3. Type of genetic modification:
Insertion;

4. In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
T-DNA borders, from pTiT37, for incorporation into plant chromosome
Trait genes used:
# a gene involved in Polyamine metabolism from Saccharomyces cerevisiae
# a gene involved in hydrolysis of proteins from Saccharomyces cerevisiae
# a cell fusion protein gene from Saccharomyces cerevisiae
# a GTP binding protein gene from Physcomitrella patens
# a gene involved in prenylation of proteins from Arabidopsis thaliana

- promoter of the 34S gene from Figwort Mosaic Virus (FMV) or promoter of the nitrilase 1 gene from Arabidopsis thaliana
- terminator of the 35S gene from the Cauliflower mosaic virus or the terminator of the nopaline synthase gene from A. tumefaciens


Selectable marker gene used:
# neomycin phosphotransferase II gene, kanamycin tolerance in plant material

- promoter and terminator from nopaline synthase gene, A. tumefaciens, gene regulation


6. Brief description of the method used for the genetic modification:
A standard Agrobacterium tumefaciens based transformation system was used for transformation of spring oilseed rape cv. Westar according to Molony et al. (1989) and Hoekema (1983).

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Seed Multiplication and evaluation of general phenotype and yield components under field conditions and selection of lines for further testing and analysis of the trait.

2. Geographical location of the site:
Municipalities of Vara, Klippan, Kristianstad, Svalöv and Eslöv.

3. Size of the site (m2):
The total trial area will not exceed 15 ha (150000 m2) in total over the five years.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Three out of the five genes of interest have been previously grown in field releases with genetically modified oilseed rape lines in Manitoba, Canada 2005 (05-BAS1-037). No adverse impacts on the environment or human health have been recorded.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified oilseed rape lines are expected to show an improved biomass production under drought and improved seed yield and quality. No difference regarding persistence or invasiveness in natural habitats compared to conventional spring oilseed rape is expected. It is expected that a selective advantage is conferred to the GM lines via the introduced trait under agricultural production. Due to the measures to be taken during the release (distance to or absence of cultivated and wild relatives, use of insect proof tents) the potential for gene transfer will be virtually excluded. Interactions of the GM oilseed rape lines with non-target species and resulting effects will be comparable to those of conventional oilseed rape varieties. The potential of increased seed yield resulting in any effects on animal or human health from contact or consumption is considered negligible. Measures in place under current field trials in relation to handling, harvest, storing, transport or disposition will further minimize the possibility of any adverse effects on health or environment. No effects are expected on biogeochemical processes from the predicted trait. The GM oilseed rape lines will be cultivated under conventional agricultural practices.

Brief description of any measures taken for the management of risks:
The distance from the nearest cultivation of oilseed rape plants will be at least 200 m. A 6m-wide protective border containing unmodified male-sterile rape will surround experimental areas. All genetically modified lines including their non-transgenic offspring will be grown under insect-proof tents. Sowing and harvesting machinery will be cleaned on site to prevent the dispersal of GM seed. Harvested seed material will be transported from the site in closed and labeled containers to the laboratories for analyses. Remaining seed will be inactivated. Vegetative plant material will be chopped and worked into the soil. The area will be controlled for volunteers for a period of 4 consecutive years. During the release period the trial site will be monitored at defined intervals.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable.

Final report

-

European Commission administrative information

Consent given by the Competent Authority: Not Known
Notification

Other notifications from this source

14,857

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